Isolation and identification of oxoproline in actinomycin X2. (V). Hydrolysates.

In the case of actinomycin X2, such reduction leads to formation of actinomycin Cx (IV, L-proline) or actinomycin X0£ (I, threohydroxy-L-proline) and Xod (allohydroxy-Lproline). Previous reports have suggested that oxoproline was destroyed by vigorous hydrolysis of actinomycin X2 with hydrochloric acid and that recovery of the imino acid was achieved in low yield after hydrolysis with hydroiodic acid at lOCFC1'2*.